Material Safety Data Sheet
Buffer Solution, pH 2.00

ACC# 03481
Section 1 - Chemical Product and Company Identification

MSDS Name: Buffer Solution, pH 2.00
Catalog Numbers: S799281, SB96-1, SB96-20, SB96-500
Synonyms: None.
Company Identification:
              Fisher Scientific
              1 Reagent Lane
              Fair Lawn, NJ 07410
For information, call: 201-796-7100
Emergency Number: 201-796-7100
For CHEMTREC assistance, call: 800-424-9300
For International CHEMTREC assistance, call: 703-527-3887

Section 2 - Composition, Information on Ingredients

CAS# Chemical Name Percent EINECS/ELINCS
7732-18-5 Water 99.43 231-791-2
7447-40-7 Potassium chloride 0.4 231-211-8
7647-01-0 Hydrochloric acid 0.10 231-595-7
50-00-0 Formaldehyde 0.05 200-001-8
67-56-1 Methyl alcohol 0.02 200-659-6

Section 3 - Hazards Identification

EMERGENCY OVERVIEW

Appearance: clear, colorless liquid.
Warning! Causes digestive and respiratory tract irritation with possible burns. Causes eye and skin irritation and possible burns.
Target Organs: Eyes, skin, mucous membranes.


Potential Health Effects
Eye: Causes eye irritation and possible burns.
Skin: May cause skin sensitization, an allergic reaction, which becomes evident upon re-exposure to this material. May cause severe irritation and possible burns.
Ingestion: May cause severe gastrointestinal tract irritation with nausea, vomiting and possible burns. May cause gastric disturbances and electrolytic imbalance.
Inhalation: May cause allergic respiratory reaction. May cause severe irritation of the respiratory tract with sore throat, coughing, shortness of breath and delayed lung edema.
Chronic: Not available.

Section 4 - First Aid Measures


Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid imme diately.
Skin: Get medical aid immediately. Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Destroy contaminated shoes.
Ingestion: Do not induce vomiting. If victim is conscious and alert, give 2-4 cupfuls of milk or water. Get medical aid immediately.
Inhalation: Get medical aid immediately. Remove from exposure and move to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen.
Notes to Physician: Treat symptomatically and supportively.

Section 5 - Fire Fighting Measures


General Information: As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. Substance is nonflammable. Use water with caution and in flooding amounts. May react exothermically with water, causing liberation of heat and possible spattering of material.
Extinguishing Media: Use water fog, dry chemical, carbon dioxide, or regular foam.
Flash Point: Not applicable.
Autoignition Temperature: Not applicable.
Explosion Limits, Lower:Not available.
Upper: Not available.
NFPA Rating: (estimated) Health: 2; Flammability: 0; Instability: 0

Section 6 - Accidental Release Measures


General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks: Absorb spill with inert material (e.g. vermiculite, sand or earth), then place in suitable container. Absorb the liquid and scrub the area with detergent and water. Clean up spills immediately, observing precautions in the Protective Equipment section.

Section 7 - Handling and Storage


Handling: Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Use with adequate ventilation. Avoid contact with eyes, skin, and clothing. Keep container tightly closed. Avoid ingestion and inhalation.
Storage: Store in a cool, dry place. Keep container closed when not in use. Do not store in metal containers.

Section 8 - Exposure Controls, Personal Protection


Engineering Controls: Use adequate ventilation to keep airborne concentrations low.
Exposure Limits
Chemical Name ACGIH NIOSH OSHA - Final PELs
Water none listed none listed none listed
Potassium chloride none listed none listed none listed
Hydrochloric acid 2 ppm Ceiling 50 ppm IDLH 5 ppm Ceiling; 7 mg/m3 Ceiling
Formaldehyde 0.3 ppm Ceiling 0.016 ppm TWA 20 ppm IDLH 0.75 ppm TWA; 2 ppm STEL; 0.5 ppm Action Level (Irritant and potential cancer ha zard - see 29 CFR 1910.1048)
Methyl alcohol 200 ppm TWA; 250 ppm STEL; Skin - potential significant contribution to overall exposure by the cutaneous r oute 200 ppm TWA; 260 mg/m3 TWA 6000 ppm IDLH 200 ppm TWA; 260 mg/m3 TWA

OSHA Vacated PELs: Water: No OSHA Vacated PELs are listed for this chemical. Potassium chloride: No OSHA Vacated PELs are listed for this chemical. Hydrochloric acid: No OSHA Vacated PELs are listed for this chemical. Formaldehyde: 3 ppm TWA (unless specified in 1910.1048) Methyl alcohol: 200 ppm TWA; 260 mg/m3 TWA
Personal Protective Equipment
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin: Wear appropriate gloves to prevent skin exposure.
Clothing: Wear appropriate protective clothing to minimize contact with skin.
Respirators: Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced.

Section 9 - Physical and Chemical Properties


Physical State: Liquid
Appearance: clear, colorless
Odor: odorless
pH: 2.00
Vapor Pressure: Not available.
Vapor Density: 0.7
Evaporation Rate:1.0
Viscosity: Not available.
Boiling Point: 212 deg F
Freezing/Melting Point:32 deg F
Decomposition Temperature:Not available.
Solubility: Soluble in water
Specific Gravity/Density:1.0
Molecular Formula:Mixture
Molecular Weight:Not available.

Section 10 - Stability and Reactivity


Chemical Stability: Stable under normal temperatures and pressures.
Conditions to Avoid: None reported.
Incompatibilities with Other Materials: None reported. Contains hydrochloric acid, which may be corrosive to metals.
Hazardous Decomposition Products: None.
Hazardous Polymerization: Has not been reported.

Section 11 - Toxicological Information


RTECS#:      
CAS# 7732-18-5: ZC0110000      
CAS# 7447-40-7: TS8050000      
CAS# 7647-01-0: MW4025000; MW4031000      
CAS# 50-00-0: LP8925000      
CAS# 67-56-1: PC1400000
LD50/LC50:
CAS# 7732-18-5:
     Oral, rat: LD50 = >90 mL/kg;
.

CAS# 7447-40-7:
     Draize test, rabbit, eye: 500 mg/24H Mild;
     Oral, mouse: LD50 = 1500 mg/kg;
     Oral, rat: LD50 = 2600 mg/kg;
.

CAS# 7647-01-0:
     Inhalation, mouse: LC50 = 1108 ppm/1H;
     Inhalation, mouse: LC50 = 20487 mg/m3/5M;
     Inhalation, mouse: LC50 = 3940 mg/m3/30M;
     Inhalation, mouse: LC50 = 8300 mg/m3/30M;
     Inhalation, rat: LC50 = 3124 ppm/1H;
     Inhalation, rat: LC50 = 60938 mg/m3/5M;
     Inhalation, rat: LC50 = 7004 mg/m3/30M;
     Inhalation, rat: LC50 = 45000 mg/m3/5M;
     Inhalation, rat: LC50 = 8300 mg/m3/30M;
     Oral, rabbit: LD50 = 900 mg/kg;
.

CAS# 50-00-0:
     Draize test, rabbit, eye: 750 ug/24H Severe;
     Draize test, rabbit, eye: 750 ug Severe;
     Draize test, rabbit, eye: 10 mg Severe;
     Draize test, rabbit, eye: 37% Severe;
     Draize test, rabbit, skin: 2 mg/24H Severe;
     Draize test, rabbit, skin: 50 mg/24H Moderate;
     Inhalation, mouse: LC50 = 454 mg/m3/4H;
     Inhalation, mouse: LC50 = 505 mg/m3/2H;
     Inhalation, rat: LC50 = 203 mg/m3;
     Inhalation, rat: LC50 = 578 mg/m3/2H;
     Inhalation, rat: LC50 = 250 ppm/2H;
     Oral, mouse: LD50 = 42
CAS# 67-56-1:
     Draize test, rabbit, eye: 40 mg Moderate;
     Draize test, rabbit, eye: 100 mg/24H Moderate;
     Draize test, rabbit, skin: 20 mg/24H Moderate;
     Inhalation, rabbit: LC50 = 81000 mg/m3/14H;
     Inhalation, rat: LC50 = 64000 ppm/4H;
     Oral, mouse: LD50 = 7300 mg/kg;
     Oral, rabbit: LD50 = 14200 mg/kg;
     Oral, rat: LD50 = 5600 mg/kg;
     Skin, rabbit: LD50 = 15800 mg/kg;
.

Carcinogenicity:
CAS# 7732-18-5: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 7447-40-7: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 7647-01-0: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 50-00-0:

CAS# 67-56-1: Not listed by ACGIH, IARC, NTP, or CA Prop 65.

Epidemiology: ~~Methanol has been shown to prooduce fetoxicity in the embryo or fetus in laboratory animals. Specific developmental abnormalities include:musculoskeletal, urogenital and cardiovascular systems,
Teratogenicity: Effects on the newborn by exposure to formaldehyde have been reported in rats by inhalation, such as behavioral effects, biochemical and metabolic effects and reduced weight gain. Cytological changes were also noted. Specific developmental abnormalities were reported by the intraperitoneal route of exposure in mice.
Reproductive Effects: Paternal effects were reported in male rats by inhalation, oral ingestion and intraperitoneal injection of formaldehyde. Organs affected included the prostate, seminal vesicle, Cowpers gland, urethra, testes, sperm duct and epididymis. Spermatogenesis was also affected.
Mutagenicity: No data available.
Neurotoxicity: No data available.
Other Studies:

Section 12 - Ecological Information


Ecotoxicity: No data available. No information available.
Environmental: No information reported.
Physical: No information available.
Other: None.

Section 13 - Disposal Considerations

Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.
RCRA P-Series: None listed.
RCRA U-Series:
CAS# 50-00-0: waste number U122.
CAS# 67-56-1: waste number U154 (Ignitable waste).

Section 14 - Transport Information

US DOT Canada TDG
Shipping Name: HYDROCHLORIC ACID No information available.
Hazard Class: 8
UN Number: UN1789
Packing Group: II

Section 15 - Regulatory Information

US FEDERAL
TSCA
     CAS# 7732-18-5 is listed on the TSCA inventory.
     CAS# 7447-40-7 is listed on the TSCA inventory.
     CAS# 7647-01-0 is listed on the TSCA inventory.
     CAS# 50-00-0 is listed on the TSCA inventory.
     CAS# 67-56-1 is listed on the TSCA inventory.
Health & Safety Reporting List
     None of the chemicals are on the Health & Safety Reporting List.
Chemical Test Rules
     None of the chemicals in this product are under a Chemical Test Rule.
Section 12b
     None of the chemicals are listed under TSCA Section 12b.
TSCA Significant New Use Rule
     None of the chemicals in this material have a SNUR under TSCA.
CERCLA Hazardous Substances and corresponding RQs
     CAS# 7647-01-0: 5000 lb final RQ; 2270 kg final RQ      CAS# 50-00-0: 100 lb final RQ; 45.4 kg final RQ      CAS# 67-56-1: 5000 lb final RQ; 2270 kg final RQ
SARA Section 302 Extremely Hazardous Substances
     CAS# 7647-01-0: 500 lb TPQ      CAS# 50-00-0: 500 lb TPQ
SARA Codes
     CAS # 7447-40-7: immediate.
     CAS # 7647-01-0: immediate.
     CAS # 50-00-0: immediate, delayed.
     CAS # 67-56-1: immediate, fire.
Section 313
     This material contains Potassium chloride (listed as Water Dissociable Nitrate Compounds), 0.4%, (CAS# 7447-40-7) which is subject to the reporting requirements of Section 313 of SARA Title III and 40 CFR Part 373.
     Hydrochloric acid is not at a high enough concentration to be reportable under Section 313.
     Formaldehyde is not at a high enough concentration to be reportable under Section 313.
     Methyl alcohol is not at a high enough concentration to be reportable under Section 313.
Clean Air Act:
     CAS# 7647-01-0 is listed as a hazardous air pollutant (HAP).
     CAS# 50-00-0 is listed as a hazardous air pollutant (HAP).
     CAS# 67-56-1 is listed as a hazardous air pollutant (HAP).
     This material does not contain any Class 1 Ozone depletors.
     This material does not contain any Class 2 Ozone depletors.
Clean Water Act:
CAS# 7647-01-0 is listed as a Hazardous Substance under the CWA. CAS# 50-00-0 is listed as a Hazardous Substance under the CWA.
     None of the chemicals in this product are listed as Priority Pollutants under the CWA.
     None of the chemicals in this product are listed as Toxic Pollutants under the CWA.
OSHA:
     CAS# 7647-01-0 is considered highly hazardous by OSHA.      CAS# 50-00-0 is considered highly hazardous by OSHA.
STATE
     CAS# 7732-18-5 is not present on state lists from CA, PA, MN, MA, FL, or NJ.
     CAS# 7447-40-7 is not present on state lists from CA, PA, MN, MA, FL, or NJ.
     CAS# 7647-01-0 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts.
     CAS# 50-00-0 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts.
     CAS# 67-56-1 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts.

California Prop 65
WARNING: This product contains Formaldehyde, a chemical known to the state of California to cause cancer.
California No Significant Risk Level: CAS# 50-00-0: 40 æg/day NSRL

European/International Regulations
European Labeling in Accordance with EC Directives
Hazard Symbols:
     XI
Risk Phrases:
     R 36/37/38 Irritating to eyes, respiratory system and skin.

Safety Phrases:
     S 24/25 Avoid contact with skin and eyes.

WGK (Water Danger/Protection)
     CAS# 7732-18-5: No information available.
     CAS# 7447-40-7: 1
     CAS# 7647-01-0: 1
     CAS# 50-00-0: 2
     CAS# 67-56-1: 1
Canada - DSL/NDSL
     CAS# 7732-18-5 is listed on Canada's DSL List.
     CAS# 7447-40-7 is listed on Canada's DSL List.
     CAS# 7647-01-0 is listed on Canada's DSL List.
     CAS# 50-00-0 is listed on Canada's DSL List.
     CAS# 67-56-1 is listed on Canada's DSL List.
Canada - WHMIS
     This product has a WHMIS classification of D2B.
This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations and the MSDS contains all of the information required by those regulations.
Canadian Ingredient Disclosure List
     CAS# 7447-40-7 is not listed on the Canadian Ingredient Disclosure List.
     CAS# 7647-01-0 is listed on the Canadian Ingredient Disclosure List.
     CAS# 50-00-0 is listed on the Canadian Ingredient Disclosure List.
     CAS# 67-56-1 is listed on the Canadian Ingredient Disclosure List.

Section 16 - Additional Information

MSDS Creation Date: 6/07/1999
Revision #4 Date: 10/03/2005

The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. In no event shall Fisher be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages, howsoever arising, even if Fisher has been advised of the possibility of such damages.